Journal: International Dental Journal

Article Title: MT2A-Mediated Regulation of Cuproptosis in Human Dental Pulp Cells Modulates Macrophage M1 Polarization in Pulpitis

doi: 10.1016/j.identj.2026.109506

Figure Lengend Snippet: MT2A knockdown in hDPCs cuproptosis model enhances macrophage migration and M1 polarization, A. Pearson correlation coefficients among immune cells and the MT2A in human pulp tissues from GEO database ( GSE92681 and GSE77459 ); B .Immunofluorescence staining of CD68 protein in human healthy pulp tissues (n = 3) and pulpitis tissues (n = 3); C. Transwell assays detected the migration of macrophages (n = 3); D. Flow cytometry detected the polarization of macrophages by the expression of CD80 and CD163 (n = 3).

Article Snippet: The cells were resuspended with 300 μL PBS and incubated with antibodies against human CD163 (PTM Bio) and CD80 (Affinity Biosciences) for 1 h on ice.

Techniques: Knockdown, Migration, Immunofluorescence, Staining, Flow Cytometry, Expressing

Journal: International Dental Journal

Article Title: MT2A-Mediated Regulation of Cuproptosis in Human Dental Pulp Cells Modulates Macrophage M1 Polarization in Pulpitis

doi: 10.1016/j.identj.2026.109506

Figure Lengend Snippet: MTII knockdown enhances macrophage infiltration and M1 polarization in the mouse pulpitis model, A. Transwell assays detected the migration of macrophages (n = 3); B. Flow cytometry detected the polarization of macrophages by the expression of CD80 and CD163 (n = 3); C. IF staining of MTII, F4/80 and iNOS protein (n = 5).

Article Snippet: The cells were resuspended with 300 μL PBS and incubated with antibodies against human CD163 (PTM Bio) and CD80 (Affinity Biosciences) for 1 h on ice.

Techniques: Knockdown, Migration, Flow Cytometry, Expressing, Staining

Journal: Frontiers in Immunology

Article Title: INPP5D/SHIP1-mediated immunometabolic remodeling of renal monocytes in idiopathic membranous nephropathy

doi: 10.3389/fimmu.2026.1755723

Figure Lengend Snippet: SHIP1-related macrophage polarization and downstream signaling in PHN kidneys. (A) Representative immunofluorescence images showing M2 (CD163) macrophage marker proteins in rat glomeruli. (B) Immunofluorescence co-staining revealed that the M1 marker iNOS and SHIP1 were co-localized (indicated by the red arrow). (C) Western blot analysis of M1- and M2-associated macrophage marker proteins in renal tissue. (D) Western blot analysis of downstream components of SHIP1 signaling, including PI3K, AKT and their phosphorylated forms. *P<0.05; ****P<0.0001. ns, no statistical significance.

Article Snippet: After blocking with normal serum, sections were incubated with primary antibodies against CD163 (Abmart, TD8235S, China) and synaptopodin (Santa Cruz, sc-515842, US) followed by fluorescently labeled secondary antibodies.

Techniques: Immunofluorescence, Marker, Staining, Western Blot

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Biofabrication of 3D-printed, pre-cross-linked alginate dialdehyde–gelatin (ADA–GEL) scaffolds for an in vivo metastatic arteriovenous loop tumor model

doi: 10.3389/fbioe.2025.1657653

Figure Lengend Snippet: CD163-stained histological cross-sections of representative animals highlighting M2 macrophage-infiltrated areas of chamber explants (red color indicates CD163-positive cells) (A) group A and (B) group B; scale bars = 50 µm.

Article Snippet: Anti-inflammatory macrophages were stained using a mouse monoclonal antibody against CD163 (1:300, clone ED2, MCA342GA, BioRad, diluted with AB Diluent, final concentration of 1.67 μg mL −1 ) and the Fast Red TR/Naphthol AS Kit (F4648-50SET, Sigma-Aldrich).

Techniques: Staining

Journal: Microorganisms

Article Title: Human Herpesvirus 6 Activates NF-κB Signalling and CD163-Positive Macrophage Recruitment in Alcohol-Induced Hepatic Injury

doi: 10.3390/microorganisms13092204

Figure Lengend Snippet: Distribution of liver lobules displaying HHV-6 positivity and CD163-positive cells across the study groups. ( a ) Proportional distribution of HHV-6-positive and HHV-6-negative liver lobules in control, young alcohol user, and long-term (chronic) alcohol user groups, providing a comparative visualisation of HHV-6 positivity across all study groups. The corresponding number of lobules is given in parentheses. ( b ) CD163-positive cell counts in the control group and chronic alcohol user (chronic alco) group, with a distinction between HHV-6-positive and negative samples. Between-group comparisons were performed using Fisher’s (F) exact test ( a ) or the non-parametric Kruskal–Wallis (KW) test with the Benjamini–Krieger–Yekutieli (BKY) two-stage linear step-up procedure as the post hoc adjustment ( b ). Boxes represent the median with interquartile range (IQR) ( b ).

Article Snippet: Specimens were stained using the standard haematoxylin and eosin (H&E) procedure and immunohistochemically labelled with a monoclonal mouse antibody against CD163 (1:50; MRQ-26, Cell Marque, Rocklin, CA, USA), a polyclonal rabbit antibody against NF-κB p65 (1:100; ab16502, Abcam, Cambridge, UK), and a mouse monoclonal antibody HHV-6 (20) against viral lysate (1:200; sc-57804, Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Control

Journal: Microorganisms

Article Title: Human Herpesvirus 6 Activates NF-κB Signalling and CD163-Positive Macrophage Recruitment in Alcohol-Induced Hepatic Injury

doi: 10.3390/microorganisms13092204

Figure Lengend Snippet: Representative microphotographs of liver tissue immunolabelled using anti-HHV-6, anti-CD163, and anti-NF-κB antibodies in all three analysed study groups—control group, young alcohol user group and chronic alcohol user group. A panel of the 1st row shows increasing HHV-6 expression as well as progressive tissue injury across study groups. A panel of the 2nd row shows a few CD163-positive cells in the control group, with increasing counts in both young alcohol user and chronic alcohol user groups, accompanied by progressive liver tissue injury across study groups. A panel of the 3rd row shows the increasing intensity of NF-κB immunoexpression across all study groups, with similar distribution patterns and progressive liver tissue injury.

Article Snippet: Specimens were stained using the standard haematoxylin and eosin (H&E) procedure and immunohistochemically labelled with a monoclonal mouse antibody against CD163 (1:50; MRQ-26, Cell Marque, Rocklin, CA, USA), a polyclonal rabbit antibody against NF-κB p65 (1:100; ab16502, Abcam, Cambridge, UK), and a mouse monoclonal antibody HHV-6 (20) against viral lysate (1:200; sc-57804, Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Control, Expressing

Journal: Scientific Reports

Article Title: ADAPT-3D:accelerated deep adaptable processing of tissue for 3-dimensional fluorescence tissue imaging for research and clinical settings

doi: 10.1038/s41598-025-16766-z

Figure Lengend Snippet: Effect of ADAPT-3D on finicky antigens and compatibility with deep immunolabeling. A ) Maximum intensity projections of tight junctions (arachnoid barrier: occludin in red and claudin-11 in green, endothelial-cell specific: claudin-5 in grey) found in leptomeninges from a wildtype mouse imaged by confocal microscopy. B ) Extended display showing en face and x-z projections of mouse ileum that was immunolabeled with alpha smooth muscle actin (yellow), lymphatic vasculature (LYVE-1, magenta), myeloid cells (S100A9, cyan), and nuclei (DAPI, grey) followed by imaging with confocal microscopy. C ) Extended display showing en face and x-z projections of ileum from a 16-week-old mouse that expresses TNF ΔARE , a model of ileitis. D ) Extended display showing en face, z-side, and 3D projections of fixed human ileum applied with decolorization, delipidation, immunolabeling with CD163 (green), IBA1 (red), and nuclei (DAPI, grey) followed by refractive index matching.

Article Snippet: 1 cm 3 samples were rinsed in 1X PBS-H for 2 h, incubated in ADAPT:DC for 2 days, ADAPT:PDL for 1 day, followed by ADAPT:BS containing antibodies against CD163 (Clone EDHu-1, Bio-Rad, 1:100), IBA1 (Fujifilm Wako, 019-19741, 1:100), and DAPI (Sigma-Aldrich, D9542, 1:200) for 2 days.

Techniques: Immunolabeling, Confocal Microscopy, Imaging, Refractive Index